Determination of Urinary Total Protein by Use of Gel Filtration and a Modified Biuret Method
نویسندگان
چکیده
منابع مشابه
Determination of total urinary protein by combined gel filtration and automated biuret reaction.
Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...
متن کاملGel filtration. Biuret method for determination of total protein in cerebrospinal fluid.
Interfering low molecular weight substances were removed from cerebro spinal fluid by gel filtration using Sephadex G-50-80 and total protein concen tration of the effluent was determined by the biuret reaction. Results of the proposed method were higher than comparable results obtained by a trichloro acetic acid turbidimetric method. Reproducibility of the method is acceptable.
متن کاملCombined gel filtration, biuret/copper method compared with an immunochemical method for urinary protein measurement.
We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were an...
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چکیده هدف از این کار بررسی الکترواکسیداسیون کتکول و مشتقات آن در حضور 4-فنیل سمی کاربامازید بوده است اکسیداسیون کتکولها ترکیبات نا پایدار کینونها را تولید می کنند که این ترکیبات می تواند در واکنش مایکل بعنوان پذیرنده نوکلئوفیل عمل نمایند. در ادامه اکسایش کتکولهای (a-c1) را درحضور 4-فنیل سمی کاربامازید در محلول آب/استونیتریل (90/10)بوسیله ولتامتری چرخه ای و کولن متری در پتانسیل ثابت مورد بررسی ...
15 صفحه اولDetermination of protein in urine by the biuret method.
A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1975
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/21.6.778